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Human TLR2 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line

Name	Human TLR2 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line
Supplier	Novus Biologicals
Catalog	NBP2-26274
Category	Cell Line
Prices	$1,129.00
Sizes	1 vial
Applications	FC
Gene	TLR2
Species	Human
Growth Mode	Adherent Morphology: Epithelial
Description	The TLR2 reporter line can be used for TLR2-dependent functional assays as well as screening of TLR2 agonists or antagonists. The TLR2 reporter cell line has been validated by flow cytometry (fig. 3) and ligand dose response assay (Fig. 2 and Fig. 3). Contents: 3~4 x 10^6 cells Biosafety Level: 2
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Product images

Flow Cytometry: Human TLR2 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26274] - Cell surface expression of TLR2 on the TLR2 reporter line was analyzed by flow cytometry using a PE-conjugated TLR2 antibody. Flow samples were prepared using the Cell Surface TLR Staining Flow Kit. Purple: Cells alone; Green: NF-kB/ SEAPorter™ HEK 293 cell line stained with anti-TLR2-PE; Red: TLR2 reporter cell line stained with anti-TLR2-PE.

Ligand Activation: Human TLR2 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26274] - Evaluation of the functional activity of the TLR2/NF-kB SEAPorterTM HEK 293 cell line by ligand dose response assay. TLR2/NF-kB SEAPorterTM HEK 293 cells were plated in 96-well plates at 5 x 10^4 cells/well. After 16 h, cells were stimulated with various amount of Pam3CSK4 for 24 h. SEAP was analyzed using the SEAPorter Assay Kit. A. Pam3CSK4-mediated TLR2 activation, as measured by SEAP activity, was increased in a dose dependent manner. B. The values from (A) were used to determine the EC50 of Pam3CSK4. The EC50, or the half maximal activity concentration, represents the concentration of Pam3CSK4 that was required for 50% activation of TLR2 as measured by SEAP activity.

Ligand Activation: Human TLR2 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26274] - Evaluation of the functional activity of the TLR2/NF-kB SEAPorterTM HEK 293 cell line by ligand dose response assay. TLR2/NF-kB SEAPorterTM HEK 293 cells were plated in 96-well plates at 5 x 10^4 cells/well. After 16 h, cells were stimulated with various amount of MALP-2 for 24 h. SEAP was analyzed using the SEAPorter Assay Kit. A. MALP2-mediated TLR2 activation, as measured by SEAP activity, was increased in a dose dependent manner. B. The values from (A) were used to determine the EC50 of MALP-2. The EC50, or the half maximal activity concentration, represents the concentration of MALP-2 that was required for 50% activation of TLR2 as measured by SEAP activity.